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(2020) Altered expression of a high quality Manage protease in E. coli reshapes the in vivo mutational landscape of the product enzyme
Protein mutational landscapes are formed by the cellular ecosystem, but key elements and their quantitative effects in many cases are unknown. Here we clearly show that Lon, a quality Command protease By natural means absent in popular E. coli expression strains, considerably reshapes the mutational landscape with the metabolic enzyme dihydrofolate reductase (DHFR). variety underneath ailments that take care of really active mutants reveals that 23.
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